Journal: Cellular & Molecular Biology Letters

Article Title: DNA dioxygenase TET2 deficiency aggravates sepsis-induced acute lung injury by targeting ITGA10 via the PI3K/AKT signaling pathway

doi: 10.1186/s11658-025-00739-1

Figure Lengend Snippet: TET2 regulates PI3K-AKT signaling pathway by targeting ITGA10 . A The diagram of sample preparation for RNA-seq. B Volcano plots are used to illustrate DGEs for HPMECs transfected with TET2 siRNA or NC siRNA. C KEGG enrichment analysis identifies the signaling pathways associated with TET2 regulated-genes. D Downregulated genes related to the integrin family were identified and their mRNA expression was validated by RT-qPCR. E Immunoblot was used to measure the protein level of ITGA10 with TET2 knockdown or overexpression. β-actin served as internal control. F Luciferase reporter assay was used to examine the relative luciferase activities in HEK 293 T cells. G MSP was used to detect the methylation level of ITGA10 promoter after TET2 overexpression. H Loci-specific 5-hmC qPCR was used to detect the abundance of 5-hmC in ITGA10 promoter after TET2 overexpression. I The protein levels of total and phosphorylated AKT and PI3K were analyzed by immunoblot with TET2 knockdown or overexpressed in vivo and in vitro. Data were expressed as mean ± standard deviation (mean ± SD). An unpaired Student’s t -test was performed to compare data between two groups. One-way ANOVA with Tukey’s multiple comparisons test was used to compare multi-group data; n = 3–4 each group; ns no significance; * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: A total 1 × 10 5 of HEK293T cells per well were plated in 48-well plates and cultured for 24 h. When the density of cells reached about 70% with better growth, the pGL4 basic promoter plasmid or the pGL4- ITGA10 promoter plasmid (GenePharma, Shanghai, China) was applied to transfect cells by using GP-transfect-Mate (GenePharma, Shanghai, China).

Techniques: Sample Prep, RNA Sequencing, Transfection, Protein-Protein interactions, Expressing, Quantitative RT-PCR, Western Blot, Knockdown, Over Expression, Control, Luciferase, Reporter Assay, Methylation, In Vivo, In Vitro, Standard Deviation

Journal: Cellular & Molecular Biology Letters

Article Title: DNA dioxygenase TET2 deficiency aggravates sepsis-induced acute lung injury by targeting ITGA10 via the PI3K/AKT signaling pathway

doi: 10.1186/s11658-025-00739-1

Figure Lengend Snippet: Suppression of ITGA10 weakens the beneficial effect of TET2 overexpression on alleviating dysfunction of HPMECs treated with LPS. A Immunoblot was used to measure the protein expression levels of p-AKT and p-PI3K under silencing ITGA10 . β-actin served as internal control. B RT-qPCR was used to detect the level of IL-1β and IL-6 . C, D CCK-8 and EdU assay were used to detect the proliferation of HPMECs. E, F The migration ability of HPMECs under silencing ITGA10 was examined using scratch assay and Transwell migration assay. G The protein expression level of VE-cadherin under silencing ITGA10 was measured by immunoblot. TET2-OE + ITGA10-si group means the group with knockdown ITGA10 after TET2 overexpression. Data were expressed as mean ± standard deviation (mean ± SD). An unpaired Student’s t -test was performed to compare data between two groups. One-way ANOVA with Tukey’s multiple comparisons test was used to compare multi-group data; n = 3–4 each group; ns no significance; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

Article Snippet: A total 1 × 10 5 of HEK293T cells per well were plated in 48-well plates and cultured for 24 h. When the density of cells reached about 70% with better growth, the pGL4 basic promoter plasmid or the pGL4- ITGA10 promoter plasmid (GenePharma, Shanghai, China) was applied to transfect cells by using GP-transfect-Mate (GenePharma, Shanghai, China).

Techniques: Over Expression, Western Blot, Expressing, Control, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Migration, Wound Healing Assay, Transwell Migration Assay, Knockdown, Standard Deviation

Journal: Cellular & Molecular Biology Letters

Article Title: DNA dioxygenase TET2 deficiency aggravates sepsis-induced acute lung injury by targeting ITGA10 via the PI3K/AKT signaling pathway

doi: 10.1186/s11658-025-00739-1

Figure Lengend Snippet: Illustration of mechanism. TET2 deficiency can aggravate the apoptosis and inflammation of LPS-treated HPMECs. Loss of TET2 reduces the expression of ITGA10 , thereby weakening the activity of the PI3K-AKT signaling pathway, promoting dysfunction of pulmonary vascular endothelial cells and exacerbating the symptoms of sepsis-induced acute lung injury

Article Snippet: A total 1 × 10 5 of HEK293T cells per well were plated in 48-well plates and cultured for 24 h. When the density of cells reached about 70% with better growth, the pGL4 basic promoter plasmid or the pGL4- ITGA10 promoter plasmid (GenePharma, Shanghai, China) was applied to transfect cells by using GP-transfect-Mate (GenePharma, Shanghai, China).

Techniques: Expressing, Activity Assay

Journal: Journal of Clinical Medicine

Article Title: Chondrogenic and Osteogenic In Vitro Differentiation Performance of Unsorted and Sorted CD34 + , CD146 + , and CD271 + Stem Cells Derived from Microfragmented Adipose Tissue of Patients with Knee Osteoarthritis

doi: 10.3390/jcm14041184

Figure Lengend Snippet: Overview of TaqMan primer-probes used for qPCR.

Article Snippet: , ITGA10 , Integrin subunit alpha 10 , A collagen type II-binding integrin expressed in cartilage tissue [ ] , Hs01006910_m1.

Techniques: Marker, Activation Assay

Journal: Journal of Clinical Medicine

Article Title: Chondrogenic and Osteogenic In Vitro Differentiation Performance of Unsorted and Sorted CD34 + , CD146 + , and CD271 + Stem Cells Derived from Microfragmented Adipose Tissue of Patients with Knee Osteoarthritis

doi: 10.3390/jcm14041184

Figure Lengend Snippet: qPCR data of chondrogenic biomarkers of unsorted, CD34 + , CD146 + , and CD271 + stem cells from microfragmented adipose tissue. Dot plots showing the relative expression of ( A ) SOX9, ( B ) COL2A1, ( C ) ACAN, ( D ) COL1A1, and ( E ) ITGA10 of chondrogenic-induced pellets (day 21) and non-induced controls. Statistical comparisons are shown between induced and non-induced samples. ns: non-significant ( p > 0.05), *: p < 0.05, **: p < 0.01, and ***: p < 0.001. The data are shown as the mean with SD. ( A , D ) The data were not normally distributed and analyzed using the Wilcoxon matched-pairs signed rank test. ( B , C , E ) The data were normally distributed and analyzed using multiple paired t -tests.

Article Snippet: , ITGA10 , Integrin subunit alpha 10 , A collagen type II-binding integrin expressed in cartilage tissue [ ] , Hs01006910_m1.

Techniques: Expressing

Journal: Journal of Clinical Medicine

Article Title: Chondrogenic and Osteogenic In Vitro Differentiation Performance of Unsorted and Sorted CD34 + , CD146 + , and CD271 + Stem Cells Derived from Microfragmented Adipose Tissue of Patients with Knee Osteoarthritis

doi: 10.3390/jcm14041184

Figure Lengend Snippet: qPCR data of chondrogenic biomarkers of unsorted, CD34 + , CD146 + , and CD271 + stem cells from microfragmented adipose tissue. Dot plots showing the relative expression of ( A ) SOX9, ( B ) COL2A1, ( C ) ACAN, ( D ) COL1A1, and ( E ) ITGA10 of chondrogenic-induced pellets (day 21) and non-induced controls as a function of cell type. Statistical comparisons are shown between cell types. Within the same culture condition, cell types not labeled with the same letter are statistically significantly different from each other ( p < 0.05). The data are shown as the mean with SD. ( A , D ) The data were not normally distributed and analyzed using the Wilcoxon matched-pairs signed rank test. ( B , C , E ) The data were normally distributed and analyzed using multiple paired t -tests.

Article Snippet: , ITGA10 , Integrin subunit alpha 10 , A collagen type II-binding integrin expressed in cartilage tissue [ ] , Hs01006910_m1.

Techniques: Expressing, Labeling